CD40 monoclonal antibody, clone EA-5
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More Files
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Flow Cytometry
5 X 105 cultured Raji human tumor cells were incubated 45 minutes on ice with 80 uL of CD40 monoclonal antibody, clone EA-5 (Cat # MAB6912) at 5 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.64 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
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Specification
Product Description
Mouse monoclonal antibody raised against native CD40.
Immunogen
Native purified CD40 from murine L cells.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate)
Storage Instruction
Store at 4°C.
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Applications
Flow Cytometry
5 X 105 cultured Raji human tumor cells were incubated 45 minutes on ice with 80 uL of CD40 monoclonal antibody, clone EA-5 (Cat # MAB6912) at 5 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.64 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. -
Gene Info — CD40
Entrez GeneID
958Gene Name
CD40
Gene Alias
Bp50, CDW40, MGC9013, TNFRSF5, p50
Gene Description
CD40 molecule, TNF receptor superfamily member 5
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
B cell surface antigen CD40|B cell-associated molecule|CD40 antigen|CD40 antigen (TNF receptor superfamily member 5)|CD40 type II isoform|CD40L receptor|OTTHUMP00000031699|OTTHUMP00000031700|nerve growth factor receptor-related B-lymphocyte activation mol
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Interactome
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Pathway
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Disease
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Publication Reference
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Impact of co-blocking the costimulatory signals on immune-related genes after high-risk rabbit corneal allograft using 2nd-generation DNA sequencing technology.
Zhao HX, Li XY, Guan WY, Han XT.
Genetics and Molecular Biology 2019 Apr; 42(2):472.
Application：Func, Rabbit, Rabbit corneal allograft.
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Follicular dendritic cells inhibit apoptosis in human B lymphocytes by a rapid and irreversible blockade of preexisting endonuclease.
Lindhout E, Lakeman A, de Groot C.
The Journal of Experimental Medicine 1995 Jun; 181(6):1985.
Application：Func, Human, Human B lymphocytes.
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Cross-linking CD40 on human B cell precursors inhibits or enhances growth depending on the stage of development and the IL costimulus.
Larson AW, LeBien TW.
The Journal of Immunology 1994 Jul; 153(2):584.
Application：Flow Cyt, Human, Splenic, Bone marrow CD10+/ surface p+ B cells.
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Soluble forms of CD40 inhibit biologic responses of human B cells.
Fanslow WC, Anderson DM, Grabstein KH, Clark EA, Cosman D, Armitage RJ.
The Journal of Immunology 1992 Jul; 149(2):655.
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Impact of co-blocking the costimulatory signals on immune-related genes after high-risk rabbit corneal allograft using 2nd-generation DNA sequencing technology.
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