CD63 monoclonal antibody, clone MEM-259 (PerCP)
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More Files
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Immunofluorescence
Immunofluorescence staining of (A) human skin fibroblasts and (B) human primary fibroblasts with CD63 monoclonal antibody, clone MEM-259 (Cat # MAB0931, green) after co-incubation of living cells with human Transferrin-Dyomics 547 (red) ; cell nuclei stained with DAPI (blue) .
Immunofluorescence
Immunofluorescence staining of CD63 in human HeLa cell line using CD63 monoclonal antibody, clone MEM-259 (Cat # MAB0931, green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue) .
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Specification
Product Description
Mouse monoclonal antibody raised against native CD63.
Immunogen
Native purified CD63 from HPB-ALL T cell line.
Host
Mouse
Theoretical MW (kDa)
40-60
Reactivity
Human
Specificity
This antibody reacts with CD63 (LAMP-3), a 40-60 KDa tetraspan glycoproteinexpressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelialcells. Cell surface exposition of CD63 is usually activation-dependent.
Form
Liquid
Conjugation
PerCP
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Immunofluorescence staining of (A) human skin fibroblasts and (B) human primary fibroblasts with CD63 monoclonal antibody, clone MEM-259 (Cat # MAB0931, green) after co-incubation of living cells with human Transferrin-Dyomics 547 (red) ; cell nuclei stained with DAPI (blue) .Immunofluorescence
Immunofluorescence staining of CD63 in human HeLa cell line using CD63 monoclonal antibody, clone MEM-259 (Cat # MAB0931, green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue) .Flow Cytometry
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Gene Info — CD63
Entrez GeneID
967Gene Name
CD63
Gene Alias
LAMP-3, ME491, MLA1, OMA81H, TSPAN30
Gene Description
CD63 molecule
Omim ID
155740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. The use of alternate polyadenylation sites has been found for this gene. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq
Other Designations
CD63 antigen|CD63 antigen (melanoma 1 antigen)|granulophysin|lysosome-associated membrane glycoprotein 3|melanoma 1 antigen|melanoma-associated antigen ME491|melanoma-associated antigen MLA1|ocular melanoma-associated antigen|tetraspanin-30
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Interactome
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Pathway
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Publication Reference
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Expression of tetraspan protein CD63 activates protein-tyrosine kinase (PTK) and enhances the PTK-induced inhibition of ROMK channels.
Lin D, Kamsteeg EJ, Zhang Y, Jin Y, Sterling H, Yue P, Roos M, Duffield A, Spencer J, Caplan M, Wang WH.
The Journal of Biological Chemistry 2008 Jan; 283(12):7674.
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CD63 as a biomarker for predicting the clinical outcomes in adenocarcinoma of lung.
Kwon MS, Shin SH, Yim SH, Lee KY, Kang HM, Kim TM, Chung YJ.
Lung Cancer 2007 Mar; 57(1):46.
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The small chemical vacuolin-1 inhibits Ca(2+)-dependent lysosomal exocytosis but not cell resealing.
Cerny J, Feng Y, Yu A, Miyake K, Borgonovo B, Klumperman J, Meldolesi J, McNeil PL, Kirchhausen T.
EMBO Reports 2004 Sep; 5(9):883.
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Expression of tetraspan protein CD63 activates protein-tyrosine kinase (PTK) and enhances the PTK-induced inhibition of ROMK channels.
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