PTPRC monoclonal antibody, clone MEM-56 (PerCP)
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blotting analysis of whole cell lysate of HUT-78 (human cutaneous T cell lymphoma cell line).
Lane 1 : Immunostaining with anti-human PTPRC control antibody.
Lane 2 : Immunostaining with PTPRC monoclonal antibody, clone MEM-56 ; sample of cell lysate prepared under non-reducing conditions.
Lane 3 : Immunostaining with PTPRC monoclonal antibody, clone MEM-56 ; sample of cell lysate prepared under reducing conditions.
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Specification
Product Description
Mouse monoclonal antibody raised against native PTPRC.
Immunogen
Native purified PTPRC from human thymocytes and T lymphocytes.
Host
Mouse
Theoretical MW (kDa)
205-220
Reactivity
Human
Specificity
This antibody reacts with CD45RA, a 205-220 KDa single chain type I glycoprotein,variant of CD45 (CD45RA isoform).
Form
Liquid
Conjugation
PerCP
Isotype
IgG2b
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blotting analysis of whole cell lysate of HUT-78 (human cutaneous T cell lymphoma cell line).
Lane 1 : Immunostaining with anti-human PTPRC control antibody.
Lane 2 : Immunostaining with PTPRC monoclonal antibody, clone MEM-56 ; sample of cell lysate prepared under non-reducing conditions.
Lane 3 : Immunostaining with PTPRC monoclonal antibody, clone MEM-56 ; sample of cell lysate prepared under reducing conditions.Flow Cytometry
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Gene Info — PTPRC
Entrez GeneID
5788Gene Name
PTPRC
Gene Alias
B220, CD45, CD45R, GP180, LCA, LY5, T200
Gene Description
protein tyrosine phosphatase, receptor type, C
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq
Other Designations
CD45 antigen|T200 glycoprotein|T200 leukocyte common antigen|glycoprotein|leukocyte-common antigen|protein tyrosine phosphatase, receptor type, c polypeptide
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Interactome
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Pathway
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Disease
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Publication Reference
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Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents.
Desharnais P, Dupere-Minier G, Hamelin C, Devine P, Bernier J.
Apoptosis 2008 Feb; 13(2):197.
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Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells.
Bijian K, Zhang L, Shen SH.
Molecular Immunology 2007 May; 44(15):3682.
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Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.
Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Bukovsky A, Horejsi V.
Folia Biol (Praha) 1989 Jan; 35(5):289.
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Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents.
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