The CCF-STTG1 cell line was fixed with 5% acetic acid methanol solution for 30 minutes followed by 5% bovine serum PBS blocking. Cells were permeabilized by 0.1% Triton X PBS for 15 minutes prior to staining with GFAP monoclonal antibody, clone SB61b (CY3) (Cat # MAB5899). Cells were then fixed and the slide was mounted with DAPI-Fluormount-G.
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq