GFAP monoclonal antibody, clone SB61b
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More Files
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Immunofluorescence
The CCF-STTG1 cell line was fixed with 5% Acetic acid Methanol solution for 30 minutes followed by 5% Bovine serum PBS blocking , cells are permeabilized by 0.1% Triton X PBS for 15 mines before and stained with GFAP monoclonal antibody, clone SB61b (Cat # MAB3336) and then cells were fixed. (The cell washing buffer was 0.15% tween 20 in PBS.) Finally the slide was mounted with Fluormount-G contained DAPI.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant GFAP.
Immunogen
Recombinant protein corresponding to human GFAP.
Host
Mouse
Reactivity
Human
Specificity
Reacts with human GFAP (~ 50 KDa).
Form
Liquid
Isotype
IgG2b
Recommend Usage
Western Blot (2 ug/mL)
Immunohistochemistry (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 100 mM BBS, pH 8.0
Storage Instruction
Store at 4°C.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunofluorescence
The CCF-STTG1 cell line was fixed with 5% Acetic acid Methanol solution for 30 minutes followed by 5% Bovine serum PBS blocking , cells are permeabilized by 0.1% Triton X PBS for 15 mines before and stained with GFAP monoclonal antibody, clone SB61b (Cat # MAB3336) and then cells were fixed. (The cell washing buffer was 0.15% tween 20 in PBS.) Finally the slide was mounted with Fluormount-G contained DAPI. -
Gene Info — GFAP
Entrez GeneID
2670Gene Name
GFAP
Gene Alias
FLJ45472
Gene Description
glial fibrillary acidic protein
Gene Ontology
HyperlinkGene Summary
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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Environmental enrichment rescues DYRK1A activity and hippocampal adult neurogenesis in TgDyrk1A.
Pons-Espinal M, Martinez de Lagran M, Dierssen M.
Neurobiology of Disease 2013 Dec; 60:18.
Application：IF, Mouse, Brain.
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Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.
Brenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE, Messing A.
Nature Genetics 2001 Jan; 27(1):117.
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Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord.
Lukas Z, Draber P, Bucek J, Dráberova E, Viklicky V, Staskova Z.
The Histochemical Journal 1989 Dec; 21(12):693.
Application：IF, IHC-Fr, WB-Ti, Human, CG/343 MG cells, Human brain, Human embryo, Human spinal cord, U333 cells.
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Environmental enrichment rescues DYRK1A activity and hippocampal adult neurogenesis in TgDyrk1A.
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