Western blot analysis from cell lysates of HeLa (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with PCNA monoclonal antibody, clone k1A3 (Cat # MAB3206 ; 1 : 2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq
DNA polymerase delta auxiliary protein,OTTHUMP00000030189,OTTHUMP00000030190,cyclin