BRCA1 monoclonal antibody, clone KEN
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of BRCA1 in U-2 OS whole cell lysate using BRCA1 monoclonal antibody, clone KEN (Cat # MAB2363). 1 minute ECL Western blot analysis.
Western Blot (Cell lysate)
Western blot analysis of BRCA1 using BRCA1 monoclonal antibody, clone KEN (Cat # MAB2363).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant BRCA1.
Immunogen
Recombinant protein corresponding to amino acids 1314-1864 of human BRCA1.
Host
Mouse
Reactivity
Human
Specificity
This antibody recognizes the epitope aa 1314-1600.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Immunocytochemistry (1:100)
Western Blot (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.05% sodium azide
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of BRCA1 in U-2 OS whole cell lysate using BRCA1 monoclonal antibody, clone KEN (Cat # MAB2363). 1 minute ECL Western blot analysis.Western Blot (Cell lysate)
Western blot analysis of BRCA1 using BRCA1 monoclonal antibody, clone KEN (Cat # MAB2363).Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — BRCA1
Entrez GeneID
672Protein Accession#
P38398Gene Name
BRCA1
Gene Alias
BRCAI, BRCC1, IRIS, PSCP, RNF53
Gene Description
breast cancer 1, early onset
Omim ID
113705Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq
Other Designations
BRCA1/BRCA2-containing complex, subunit 1|breast and ovarian cancer susceptibility protein 1
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Interactome
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Pathway
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Disease
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Publication Reference
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A member of the Pyrin family, IFI16, is a novel BRCA1-associated protein involved in the p53-mediated apoptosis pathway.
Aglipay JA, Lee SW, Okada S, Fujiuchi N, Ohtsuka T, Kwak JC, Wang Y, Johnstone RW, Deng C, Qin J, Ouchi T.
Oncogene 2003 Dec; 22(55):8931.
Application：IF, IP, WB, Human, Mouse, HCC1937, HeLa, MCF10A cells, MEFs.
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Cell cycle differences in DNA damage-induced BRCA1 phosphorylation affect its subcellular localization.
Okada S, Ouchi T.
The Journal of Biological Chemistry 2003 Jan; 278(3):2015.
Application：IF, WB, Human, HCC1937, MCF-7 cells.
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A member of the Pyrin family, IFI16, is a novel BRCA1-associated protein involved in the p53-mediated apoptosis pathway.
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