EEF2 monoclonal antibody, clone 5B6
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using EEF2 monoclonal antibody, clone 5B6 (Cat # MAB10629) against HepG2 (1) , HeLa (2) , HEK293 (3) and A-431 (4) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissues (A) , kidney cancer tissues (B) , prostate using EEF2 monoclonal antibody, clone 5B6 (Cat # MAB10629) .
Immunofluorescence
Immunofluorescence analysis of 3T3-L1 cells using EEF2 monoclonal antibody, clone 5B6 (Cat # MAB10629) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of EEF2 monoclonal antibody, clone 5B6 (Cat # MAB10629) .
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant EEF2.
Immunogen
Recombinant protein corresponding to human EEF2.
Host
Mouse
Theoretical MW (kDa)
95
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using EEF2 monoclonal antibody, clone 5B6 (Cat # MAB10629) against HepG2 (1) , HeLa (2) , HEK293 (3) and A-431 (4) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissues (A) , kidney cancer tissues (B) , prostate using EEF2 monoclonal antibody, clone 5B6 (Cat # MAB10629) .Immunofluorescence
Immunofluorescence analysis of 3T3-L1 cells using EEF2 monoclonal antibody, clone 5B6 (Cat # MAB10629) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of EEF2 monoclonal antibody, clone 5B6 (Cat # MAB10629) . -
Gene Info — EEF2
Entrez GeneID
1938Gene Name
EEF2
Gene Alias
EEF-2, EF2
Gene Description
eukaryotic translation elongation factor 2
Omim ID
130610Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq
Other Designations
polypeptidyl-tRNA translocase
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Interactome
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Disease
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