CREB1 monoclonal antibody, clone 5G3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using CREB1 monoclonal antibody, clone 5G3 (Cat # MAB10612) against K-562 (1) , Jurkat (2) , L1210 (3) , HEK293 (4) , A-431 (5) , HeLa (6) , COS-7 (7) , PC-12 (8) , and NIH/3T3 (9) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues (upper) and submaxillary tumor tissues (bottom) using CREB1 monoclonal antibody, clone 5G3 (Cat # MAB10612) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using CREB1 monoclonal antibody, clone 5G3 (Cat # MAB10612) (green) . Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of CREB1 monoclonal antibody, clone 5G3 (Cat # MAB10612) .
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CREB1.
Immunogen
Recombinant protein corresponding to human CREB1.
Host
Mouse
Theoretical MW (kDa)
43
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using CREB1 monoclonal antibody, clone 5G3 (Cat # MAB10612) against K-562 (1) , Jurkat (2) , L1210 (3) , HEK293 (4) , A-431 (5) , HeLa (6) , COS-7 (7) , PC-12 (8) , and NIH/3T3 (9) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues (upper) and submaxillary tumor tissues (bottom) using CREB1 monoclonal antibody, clone 5G3 (Cat # MAB10612) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using CREB1 monoclonal antibody, clone 5G3 (Cat # MAB10612) (green) . Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of CREB1 monoclonal antibody, clone 5G3 (Cat # MAB10612) . -
Gene Info — CREB1
Entrez GeneID
1385Gene Name
CREB1
Gene Alias
CREB, MGC9284
Gene Description
cAMP responsive element binding protein 1
Omim ID
123810Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
active transcription factor CREB|cAMP-response element-binding protein-1|transactivator protein
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Interactome
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Pathway
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Disease
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