CDH1 monoclonal antibody, clone 7H12
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using CDH1 monoclonal antibody, clone 7H12 (Cat # MAB10546) against LNCAP (1)，A-431 (2), DU145 (3), PC-3 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), C6 (8) and COS-7 (9) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human gastric cancer tissues (A) and lung cancer tissues (B) using CDH1 monoclonal antibody, clone 7H12 (Cat # MAB10546) with DAB staining.
Flow Cytometry
Flow cytometric analysis of HeLa cells using CDH1 monoclonal antibody, clone 7H12 (Cat # MAB10546) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CDH1.
Immunogen
Recombinant protein corresponding to human CDH1.
Host
Mouse
Theoretical MW (kDa)
135
Reactivity
Human, Monkey, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using CDH1 monoclonal antibody, clone 7H12 (Cat # MAB10546) against LNCAP (1)，A-431 (2), DU145 (3), PC-3 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), C6 (8) and COS-7 (9) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human gastric cancer tissues (A) and lung cancer tissues (B) using CDH1 monoclonal antibody, clone 7H12 (Cat # MAB10546) with DAB staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using CDH1 monoclonal antibody, clone 7H12 (Cat # MAB10546) (green) and negative control (purple). -
Gene Info — CDH1
Entrez GeneID
999Gene Name
CDH1
Gene Alias
Arc-1, CD324, CDHE, ECAD, LCAM, UVO
Gene Description
cadherin 1, type 1, E-cadherin (epithelial)
Gene Ontology
HyperlinkGene Summary
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq
Other Designations
cadherin 1, E-cadherin (epithelial)|cadherin 1, type 1|calcium-dependent adhesion protein, epithelial|cell-CAM 120/80|uvomorulin
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Interactome
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Pathway
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Disease
- Adenocarcinoma
- Asthma
- Breast cancer
- Breast Neoplasms
- Bronchiolitis
- Carcinoma
- Cleft Lip
- Cleft Palate
- Colitis
+ View More Disease
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