Mouse monoclonal antibody raised against synthetic peptide of CD9.
Immunogen:
A synthetic peptide corresponding to human CD9.
Host:
Mouse
Theoretical MW (kDa):
25
Reactivity:
Human
Form:
Liquid
Isotype:
IgG1
Recommend Usage:
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
Storage Buffer:
In ascites (0.03% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Transfected lysate)
Western blot analysis using CD9 monoclonal antibody, clone 5G6 (Cat # MAB10542) against HEK293 (1) and CD9(aa : 37-228)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis of paraffin-embedded human cervical cancer (A) and human kidney cancer(B) tissues using CD9 monoclonal antibody, clone 5G6 (Cat # MAB10542) with DAB staining.
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance. [provided by RefSeq
Other Designations:
5H9 antigen,CD9 antigen,CD9 antigen (p24),OTTHUMP00000041574,OTTHUMP00000041576,antigen defined by monoclonal antibody 602-29,growth-inhibiting gene 2 protein,leukocyte antigen MIC3,motility related protein,motility related protein-1,p24 antigen