BRAF monoclonal antibody, clone 1H12F1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using BRAF monoclonal antibody, clone 1H12F1 (Cat # MAB10455) against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Braf cDNA (2).
Western Blot
Western blot analysis using BRAF monoclonal antibody, clone 1H12F1 (Cat # MAB10455) against truncated recombinant BRAF (1) and A-431 cell lysate (2).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human testis tissue using BRAF monoclonal antibody, clone 1H12F1 (Cat # MAB10455).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue(A) and lung carcinoma tissue (B) showing cytoplasmic localization using BRAF monoclonal antibody, clone 1H12F1 (Cat # MAB10455) with DAB staining.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant BRAF.
Immunogen
Recombinant protein corresponding to human BRAF.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascitic (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using BRAF monoclonal antibody, clone 1H12F1 (Cat # MAB10455) against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Braf cDNA (2).Western Blot
Western blot analysis using BRAF monoclonal antibody, clone 1H12F1 (Cat # MAB10455) against truncated recombinant BRAF (1) and A-431 cell lysate (2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human testis tissue using BRAF monoclonal antibody, clone 1H12F1 (Cat # MAB10455).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue(A) and lung carcinoma tissue (B) showing cytoplasmic localization using BRAF monoclonal antibody, clone 1H12F1 (Cat # MAB10455) with DAB staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — BRAF
Entrez GeneID
673Gene Name
BRAF
Gene Alias
B-RAF1, BRAF1, FLJ95109, MGC126806, MGC138284, RAFB1
Gene Description
v-raf murine sarcoma viral oncogene homolog B1
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq
Other Designations
94 kDa B-raf protein|B-Raf proto-oncogene serine/threonine-protein kinase (p94)|Murine sarcoma viral (v-raf) oncogene homolog B1
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Interactome
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Pathway
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Disease
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