SMAD4 monoclonal antibody, clone 4G1C6
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant SMAD4.
Immunogen
Recombinant protein corresponding to human SMAD4.
Host
Mouse
Theoretical MW (kDa)
65
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of SMAD4 monoclonal antobody, clone 4G1C6 (Cat # MAB10392) against A-431 (1), SK-N-SH (2), K-562 (3), HepG2 (4) and HUVE12 (5) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using SMAD4 monoclonal antobody, clone 4G1C6 (Cat # MAB10392)Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using SMAD4 monoclonal antobody, clone 4G1C6 (Cat # MAB10392) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of K-562 cells using SMAD4 monoclonal antobody, clone 4G1C6 (Cat # MAB10392) (green) and negative control (purple). -
Gene Info — SMAD4
Entrez GeneID
4089Gene Name
SMAD4
Gene Alias
DPC4, JIP, MADH4
Gene Description
SMAD family member 4
Gene Ontology
HyperlinkGene Summary
mothers against decapentaplegic homolog 4|OTTHUMP00000163548|SMAD
Other Designations
MAD, mothers against decapentaplegic homolog 4|OTTHUMP00000163548|SMAD, mothers against DPP homolog 4|deleted in pancreatic carcinoma locus 4|mothers against decapentaplegic homolog 4|mothers against decapentaplegic, Drosophila, homolog of, 4
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Interactome
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Pathway
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Disease
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