Mouse monoclonal antibody raised against recombinant SERPINE1.
Immunogen:
Recombinant protein corresponding to human SERPINE1.
Host:
Mouse
Theoretical MW (kDa):
45
Reactivity:
Human
Form:
Liquid
Recommend Usage:
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (0.5% protein stabilizer, 0.05% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Transfected lysate)
Western blot analysis using SERPINE1 monoclonal antobody, clone 1D5 (Cat # MAB10390) against HEK293 (1) and SERPINE1 (AA: 194-316)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using SERPINE1 monoclonal antobody, clone 1D5 (Cat # MAB10390) against human SERPINE1 (aa 194-316) recombinant protein. (Expected MW is 45kDa kDa)
Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and human kidney cancer (B) tissue using SERPINE1 monoclonal antobody, clone 1D5 (Cat # MAB10390) with DAB staining.
ELISA
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using SERPINE1 monoclonal antobody, clone 1D5 (Cat # MAB10390) (green) and negative control (red).
This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations:
plasminogen activator inhibitor, type I,plasminogen activator inhibitor-1,serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1