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Last updated: 2014/10/20
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UBB monoclonal antibody, clone 3C12

  • Catalog # : MAB10342
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant UBB.
  • Immunogen:
  • Recombinant protein corresponding to human UBB.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 26
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.5% protein stabilizer, 0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using UBB monoclonal antobody, clone 3C12 (Cat # MAB10342) against NIH/3T3 (1) and HeLa (2) cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using UBB monoclonal antobody, clone 3C12 (Cat # MAB10342) against recombinant human UBB protein.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using UBB monoclonal antobody, clone 3C12 (Cat # MAB10342) (green) and negative control (red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 7314
  • Gene Name:
  • UBB
  • Gene Alias:
  • FLJ25987,MGC8385
  • Gene Description:
  • ubiquitin B
  • Gene Summary:
  • This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000064960,OTTHUMP00000064961,polyubiquitin B
  • Gene Pathway
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