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Last updated: 2014/11/16
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TNNI2 monoclonal antibody, clone 2F12A11

  • Catalog # : MAB10337
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant TNNI2.
  • Immunogen:
  • Recombinant protein corresponding to human TNNI2.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 21
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis using TNNI2 monoclonal antobody, clone 2F12A11 (Cat # MAB10337) against HEK293 (1) and TNNI2-hIgGFc transfected HEK293 (2) cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using TNNI2 monoclonal antobody, clone 2F12A11 (Cat # MAB10337) against recombinant human TNNI2 protein.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human rabbit cardiac muscle tissue (A), cardiac muscle tissue (B) and striated muscle tissue (C) using TNNI2 monoclonal antobody, clone 2F12A11 (Cat # MAB10337) with DAB staining.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of NIH/3T3 cells using TNNI2 monoclonal antobody, clone 2F12A11 (Cat # MAB10337) (blue) and negative control (red).
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 7136
  • Gene Name:
  • TNNI2
  • Gene Alias:
  • AMCD2B,DA2B,FSSV
  • Gene Description:
  • troponin I type 2 (skeletal, fast)
  • Gene Summary:
  • This gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies show that this component is also present in vascular smooth muscle and may play a role in regulation of smooth muscle function. In addition to muscle tissues, this protein is found in corneal epithelium, cartilage where it is an inhibitor of angiogenesis to inhibit tumor growth and metastasis, and mammary gland where it functions as a co-activator of estrogen receptor-related receptor alpha. This protein also suppresses tumor growth in human ovarian carcinoma. Mutations in this gene cause myopathy and distal arthrogryposis type 2B. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000014141,OTTHUMP00000014142,troponin I fast twitch 2,troponin I, fast-twitch skeletal muscle isoform,troponin I, skeletal, fast
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