Mouse monoclonal antibody raised against native FAS.
Native purified FAS from human T cell lymphoma cell line HUT-78.
The optimal working dilution should be determined by the end user.
In PBS, pH 7.4
Store at 4°C. Do not freeze.
Induction of apoptosis in various hematopoetic cell lines by use of FAS monoclonal antibody, clone UT - 1 (Cat # MAB0958). Suspensions of used cell lines were incubated with soluble (or without as control) FAS monoclonal antibody, clone UT - 1. Induction of apoptosis was measured by Apoptosis Assay Kit - FITC. Histograms : Red (full), untreated control cells. Black, standard apoptosis - inducing anti - Fas antibody. Green, FAS monoclonal antibody, clone UT - 1 (Cat # MAB0958). A : Jurkat human peripheral blood T cell leukemia cell line. B : TF - 1 human bone marrow erythroleukemia cell line. C : CEM human leukemia cell line. D : MOLT - 4 human acute lymphoblastic T cell leukemia cell line.
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq
APO-1 cell surface antigen,CD95 antigen,Fas AMA,Fas antigen,OTTHUMP00000020045,OTTHUMP00000020046,OTTHUMP00000020051,OTTHUMP00000059646,apoptosis antigen 1,tumor necrosis factor receptor superfamily member 6,tumor necrosis factor receptor superfamily, mem