COMMD1 monoclonal antibody (M01), clone 2A12
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More Files
- More Functions
Western Blot (Cell lysate)
COMMD1 monoclonal antibody (M01), clone 2A12. Western Blot analysis of COMMD1 expression in HepG2.
Western Blot (Transfected lysate)
Western Blot analysis of COMMD1 expression in transfected 293T cell line by COMMD1 monoclonal antibody (M01), clone 2A12.
Lane 1: COMMD1 transfected lysate(21.2 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of COMMD1 transfected lysate using anti-COMMD1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with COMMD1 MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged COMMD1 is approximately 0.03ng/ml as a capture antibody.
Western Blot detection against Immunogen (46.64 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant COMMD1.
Immunogen
COMMD1 (AAH22046.1, 1 a.a. ~ 190 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAAGELEGGKPLSGLLNALAQDTFHGYPGITEELLRSQLYPEVPPEEFRPFLAKMRGILKSIASADMDFNQLEAFLTAQTKKQGGITSDQAAVISKFWKSHKTKIRESLMNQSRWNSGLRGLSWRVDGKSQSRHSAQIHTPVAIIELELGKYGQESEFLCLEFDEVKVNQILKTLSEVEESISTLISQPN
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (46.64 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
COMMD1 monoclonal antibody (M01), clone 2A12. Western Blot analysis of COMMD1 expression in HepG2.Western Blot (Transfected lysate)
Western Blot analysis of COMMD1 expression in transfected 293T cell line by COMMD1 monoclonal antibody (M01), clone 2A12.
Lane 1: COMMD1 transfected lysate(21.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of COMMD1 transfected lysate using anti-COMMD1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with COMMD1 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged COMMD1 is approximately 0.03ng/ml as a capture antibody.ELISA
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Gene Info — COMMD1
Entrez GeneID
150684GeneBank Accession#
BC022046Protein Accession#
AAH22046.1Gene Name
COMMD1
Gene Alias
C2orf5, MGC27155, MURR1
Gene Description
copper metabolism (Murr1) domain containing 1
Omim ID
607238Gene Ontology
HyperlinkGene Summary
COMMD1 is a regulator of copper homeostasis, sodium uptake, and NF-kappa-B (see MIM 164011) signaling (de Bie et al., 2005 [PubMed 16267171]).[supplied by OMIM
Other Designations
MURR1|copper metabolism gene MURR1
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Interactome
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Disease
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Publication Reference
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The Anticancer Peptide CIGB-552 Exerts Anti-Inflammatory and Anti-Angiogenic Effects through COMMD1.
Hellen Daghero, Julio Raúl Fernández Massó, Soledad Astrada, Maribel Guerra Vallespí, Mariela Bollati-Fogolín.
Molecules (Basel, Switzerland) 2020 Dec; 26(1):152.
Application：IF, WB-Tr, Human, HT-29, NCI-H460 cells.
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P300-mediated acetylation of COMMD1 regulates its stability and the ubiquitination/nucleolar translocation of NF-κB/RelA.
O'Hara A, Simpson J, Morin P, Loveridge CJ, Williams AC, Novo SM, Stark LA.
Journal of Cell Science 2014 Sep; 127(Pt 17):3659.
Application：WB-Ce, WB-Ti, WB-Tr, Human, SW480, AA/C1 cells, Colorectal cancer.
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Radiosensitization of normoxic and hypoxic h1339 lung tumor cells by heat shock protein 90 inhibition is independent of hypoxia inducible factor-1α.
SSchilling D, Bayer C, Li W, Molls M, Vaupel P, Multhoff G.
PLoS One 2012 Feb; 7(2):e31110.
Application：WB-Ce, Human, EPLC-272H, H1339 cells.
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COMMD1-Mediated Ubiquitination Regulates CFTR Trafficking.
Drevillon L, Tanguy G, Hinzpeter A, Arous N, de Becdelievre A, Aissat A, Tarze A, Goossens M, Fanen P.
PLoS One 2011 Mar; 6(3):e18334.
Application：IF, IP, WB-Ce, WB-Tr, Human, HeLa, HT-29 cells.
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Cu/Zn superoxide dismutase maturation and activity are regulated by COMMD1.
Vonk WI, Wijmenga C, Berger R, van de Sluis B, Klomp LW.
The Journal of Biological Chemistry 2010 Sep; 285(37):28991.
Application：IP, WB-Ce, WB-Tr, Human, HEK 293T, HepG2 cells.
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Copper-Induced Translocation of the Wilson Disease Protein ATP7B Independent of Murr1/COMMD1 and Rab7.
Weiss KH, Lozoya JC, Tuma S, Gotthardt D, Reichert J, Ehehalt R, Stremmel W, Fullekrug J.
The American Journal of Pathology 2008 Oct; 173(6):1783.
Application：IF, WB-Tr, Human, Huh7, Ptk2 cells.
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COMMD1 forms oligomeric complexes targeted to the endocytic membranes via specific interactions with PtdIns(4,5)p2.
Burkhead JL, Morgan CT, Shinde U, Haddock G, Lutsenko S.
The Journal of Biological Chemistry 2008 Oct; 284(1):696.
Application：IF, WB, Human, HepG2 cells.
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Tumor suppressor ARF promotes non-classic proteasomal independent polyubiquitination of COMMD1.
Huang Y, Wu M, Li HY.
The Journal of Biological Chemistry 2008 Feb; 283(17):11453.
Application：IF, Human, NCI-H1299 cells.
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Distinct Wilson's disease mutations in ATP7B are associated with enhanced binding to COMMD1 and reduced stability of ATP7B.
de Bie P, van de Sluis B, Burstein E, van de Berghe PV, Muller P, Berger R, Gitlin JD, Wijmenga C, Klomp LW.
Gastroenterology 2007 Jul; 133(4):1316.
Application：IF, Human, HEK 293T cells.
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COMMD1 promotes the ubiquitination of NF-kappaB subunits through a cullin-containing ubiquitin ligase.
Maine GN, Mao X, Komarck CM, Burstein E.
The EMBO Journal 2006 Dec; 26(2):436.
Application：IP-WB, WB-Tr, Human, HEK 293, NIH-SR cells.
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The Anticancer Peptide CIGB-552 Exerts Anti-Inflammatory and Anti-Angiogenic Effects through COMMD1.
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