CAMKK1 monoclonal antibody (M01), clone 1F6
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More Files
- More Functions
Western Blot (Cell lysate)
CAMKK1 monoclonal antibody (M01), clone 1F6 Western Blot analysis of CAMKK1 expression in Jurkat ( Cat # L017V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of CAMKK1 expression in transfected 293T cell line by CAMKK1 monoclonal antibody (M01), clone 1F6.
Lane 1: CAMKK1 transfected lysate(57.31 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CAMKK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CAMKK1 is approximately 1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of CAMKK1 over-expressed 293 cell line, cotransfected with CAMKK1 Validated Chimera RNAi ( Cat # H00084254-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMKK1 monoclonal antibody (M01) clone 1F6 (Cat # H00084254-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (82.94 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant CAMKK1.
Immunogen
CAMKK1 (AAH31647, 1 a.a. ~ 520 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEGGPAVCCQDPRAELVERVAAIDVTHLEEADGGPEPTRNGVDPPPRARAASVIPGSTSRLLPARPSLSARKLSLQERPAGSYLEAQAGPYATGPASHISPRAWRRPTIESHHVAISDAEDCVQLNQYKLQSEIGKGAYGVVRLAYNESEDRHYAMKVLSKKKLLKQYGFPRRPPPRGSQAAQGGPAKQLLPLERVYQEIAILKKLDHVNVVKLIEVLDDPAEDNLYLALQNQAQNIQLDSTNIAKPHSLLPSEQQDSGSTWAARSVFDLLRKGPVMEVPCDKPFSEEQARLYLRDVILGLEYLHCQKIVHRDIKPSNLLLGDDGHVKIADFGVSNQFEGNDAQLSSTAGTPAFMAPEAISDSGQSFSGKALDVWATGVTLYCFVYGKCPFIDDFILALHRKIKNEPVVFPEGPEISEELKDLILKMLDKNPETRIGVPDIKLHPWVTKNGEEPLPSEEEHCSVVEVTEEEVKNSVRLIPSWTTVILVKSMLRKRSFGNPFEPQARREERSMSAPGNLLV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (87); Rat (87)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (82.94 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CAMKK1 monoclonal antibody (M01), clone 1F6 Western Blot analysis of CAMKK1 expression in Jurkat ( Cat # L017V1 ).Western Blot (Transfected lysate)
Western Blot analysis of CAMKK1 expression in transfected 293T cell line by CAMKK1 monoclonal antibody (M01), clone 1F6.
Lane 1: CAMKK1 transfected lysate(57.31 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CAMKK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CAMKK1 is approximately 1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of CAMKK1 over-expressed 293 cell line, cotransfected with CAMKK1 Validated Chimera RNAi ( Cat # H00084254-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMKK1 monoclonal antibody (M01) clone 1F6 (Cat # H00084254-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — CAMKK1
Entrez GeneID
84254GeneBank Accession#
BC031647Protein Accession#
AAH31647Gene Name
CAMKK1
Gene Alias
CAMKKA, DKFZp761M0423, MGC34095
Gene Description
calcium/calmodulin-dependent protein kinase kinase 1, alpha
Omim ID
611411Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
CAMKK alpha protein|OTTHUMP00000115904|calcium/calmodulin-dependent protein kinase 1 alpha
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Interactome
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Pathway
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Disease
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