NDE1 monoclonal antibody (M01), clone 2G11-1C11
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant NDE1.
Immunogen
NDE1 (AAH01421, 1 a.a. ~ 335 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEDSGKTFSSEEEEANYWKDLAMTYKQRAENTQEELREFQEGSREYEAELETQLQQIETRNRDLLSENNRLRMELETIKEKFEVQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQRLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEKPRTPMPSSVEAERTDTAVQATGSVPSTPIAHRGPSSSLNTPGSFRRGLDDSTGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNLVYDQSPNRTGGPASGRSSKNRDGGERRPSSTSVPLGDKGLDTSCRWLSKSTTRSSSSC
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (90); Rat (83)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (62.59 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
NDE1 monoclonal antibody (M01), clone 2G11-1C11 Western Blot analysis of NDE1 expression in Hela ( Cat # L013V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NDE1 is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to NDE1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — NDE1
Entrez GeneID
54820GeneBank Accession#
BC001421Protein Accession#
AAH01421Gene Name
NDE1
Gene Alias
FLJ20101, HOM-TES-87, NUDE, NUDE1
Gene Description
nudE nuclear distribution gene E homolog 1 (A. nidulans)
Omim ID
609449Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization, mitosis and neuronal migration. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
LIS1-interacting protein NUDE1, rat homolog|OTTHUMP00000160329|OTTHUMP00000160330|nuclear distribution gene E homolog 1
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Interactome
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Disease
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Publication Reference
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Micronuclei from misaligned chromosomes that satisfy the spindle assembly checkpoint in cancer cells.
Ana Margarida Gomes, Bernardo Orr, Marco Novais-Cruz, Filipe De Sousa, Joana Macário-Monteiro, Carolina Lemos, Cristina Ferrás, Helder Maiato.
Current Biology : CB 2022 Aug; [Epub].
Application:WB-Tr, Human, Hela cells.
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Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.
Ana C Almeida, Joana Soares-de-Oliveira, Danica Drpic, Liam P Cheeseman, Joana Damas, Harris A Lewin, Denis M Larkin, Paulo Aguiar, António J Pereira, Helder Maiato.
Cell Reports 2022 Apr; 39(1):110610.
Application:WB-Ce, Indian muntjac, Indian muntjac fibroblasts.
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Centrosome-nuclear axis repositioning drives the assembly of a bipolar spindle scaffold to ensure mitotic fidelity.
Vanessa Nunes, Margarida Dantas, Domingos Castro, Elisa Vitiello, Irène Wang, Nicolas Carpi, Martial Balland, Matthieu Piel, Paulo Aguiar, Helder Maiato, Jorge G Ferreira.
Molecular Biology of the Cell 2020 Jul; 31(16):1675.
Application:WB-Tr, Human, HeLa cells.
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Severe NDE1-mediated microcephaly results from neural progenitor cell cycle arrests at multiple specific stages.
Doobin DJ, Kemal S, Dantas TJ, Vallee RB.
Nature Communications 2016 Aug; 7:12551.
Application:WB-Tr, Rat, Rat brain glioma cell C6.
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NDE1, NDEL1: Multimerisation, alternate splicing and DISC1 interaction.
Bradshaw NJ, Christie S, Soares DC, Carlyle BC, Porteous DJ, Millar JK.
Neuroscience Letters 2008 Nov; 449(3):228.
Application:WB, Human, HEK 293T cells.
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Micronuclei from misaligned chromosomes that satisfy the spindle assembly checkpoint in cancer cells.
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