Western blot analysis of BFAR over-expressed 293 cell line, cotransfected with BFAR Validated Chimera RNAi ( Cat # H00051283-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with BFAR monoclonal antibody (M01), clone 1C6 (Cat # H00051283-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.