SERBP1 monoclonal antibody (M01), clone 1D2-2E9
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More Files
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Western Blot (Cell lysate)
PAI-RBP1 monoclonal antibody (M01), clone 1D2-2E9 Western Blot analysis of PAI-RBP1 expression in HeLa ( Cat # L013V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of SERBP1 expression in transfected 293T cell line by PAI-RBP1 monoclonal antibody (M01), clone 1D2-2E9.
Lane 1: SERBP1 transfected lysate(45 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SERBP1 is 0.3 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to PAI-RBP1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (69.96 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant SERBP1.
Immunogen
SERBP1 (AAH02488, 1 a.a. ~ 402 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MPGHLQEGFGCVVTNRFDQLFDDESDPFEVLKAAENKKKEAGGGGVGGPGAKSAAQAAAQTNSNAAGKQLRKESQKDRKNPLPPSVGVVDKKEETQPPVALKKEGIRRVGRRPDQQLQGEGKIIDRRPERRPPRERRFEKPLEEKGEGGEFSVDRPIIDRPIRGRGGLGRGRGGRGRGMGRGDGFDSRGKREFDRHSGSDRSGLKHEDKRGGSGSHNWGTVKDELTESPKYIQKQISYNYSDLDQSNVTEETPEGEEHHPVADTENKENEVEEVKEEGPKEMTLDEWKAIQNKDRAKVEFNIRKPNEGADGQWKKGFVLHKSKSEEAHAEDSVMDHHFRKPANDITSQLEINFGDLGRPGRGGRGGRGGCGRGGRPNRGSRTDKSSASAPDVDDPEAFPALA
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (96)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (69.96 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PAI-RBP1 monoclonal antibody (M01), clone 1D2-2E9 Western Blot analysis of PAI-RBP1 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of SERBP1 expression in transfected 293T cell line by PAI-RBP1 monoclonal antibody (M01), clone 1D2-2E9.
Lane 1: SERBP1 transfected lysate(45 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SERBP1 is 0.3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PAI-RBP1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — SERBP1
Entrez GeneID
26135GeneBank Accession#
BC002488Protein Accession#
AAH02488Gene Name
SERBP1
Gene Alias
CGI-55, CHD3IP, DKFZp564M2423, FLJ90489, HABP4L, PAI-RBP1, PAIRBP1
Gene Description
SERPINE1 mRNA binding protein 1
Omim ID
607378Gene Ontology
HyperlinkOther Designations
OTTHUMP00000010977|OTTHUMP00000010978|OTTHUMP00000037681|OTTHUMP00000037682|PAI-1 mRNA binding protein|chromodomain helicase DNA binding protein 3 interacting protein|plasminogen activator inhibitor 1 RNA binding protein
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Interactome
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Publication Reference
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ETD-Based Proteomic Profiling Improves Arginine Methylation Identification and Reveals Novel PRMT5 Substrates.
Lingzi Lu, Zilu Ye, Rou Zhang, Jesper V. Olsen, Yanqiu Yuan, and Yang Mao.
Journal of Proteome Research 2024 Mar; 23(3):1014.
Application：IF, WB, Human, Hela cells.
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Silencing of Ago-2 Interacting Protein SERBP1 Relieves KCC2 Repression by miR-92 in Neurons.
Christian Barbato, Paola Frisone, Laura Braccini, Simona D'Aguanno, Luisa Pieroni, Maria Teresa Ciotti, Caterina Catalanotto, Carlo Cogoni, Francesca Ruberti.
Cells 2022 Mar; 11(6):1052.
Application：WB-Tr, Human, Rat, Rat primary hippocampal neurons, SH-SY5Y cells.
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Localization of SERBP1 in stress granules and nucleoli.
Lee YJ, Wei HM, Chen LY, Li C.
The FEBS Journal 2014 Jan; 281(1):352.
Application：IF, Human, HeLa cells.
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Protein arginine methylation of SERBP1 by protein arginine methyltransferase 1 affects cytoplasmic/nuclear distribution.
Lee YJ, Hsieh WY, Chen LY, Li C.
Journal of Cellular Biochemistry 2012 Aug; 113(8):2721.
Application：WB-Tr, Human, HeLa cells.
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Serologic Markers of Effective Tumor Immunity against Chronic Lymphocytic Leukemia Include Nonmutated B-Cell Antigens.
Marina O, Hainz U, Biernacki MA, Zhang W, Cai A, Duke-Cohan JS, Liu F, Brusic V, Neuberg D, Kutok JL, Alyea EP, Canning CM, Soiffer RJ, Ritz J, Wu CJ.
Cancer Research 2010 Feb; 70(4):1344.
Application：WB, Human, PBMC, B, CLL cells.
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An integrated approach of differential mass spectrometry and gene ontology analysis identified novel proteins regulating neuronal differentiation and survival.
Kobayashi D, Kumagai J, Morikawa T, Wilson-Morifuji M, Wilson A, Irie A, Araki N.
Molecular & Cellular Proteomics 2009 Oct; 8(10):2350.
Application：WB, Rat, PC12 cells.
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Rapid Changes of mRNA-binding Protein Levels following Glucose and 3-Isobutyl-1-methylxanthine Stimulation of Insulinoma INS-1 Cells.
Sus C, Czupalla C, Winter C, Pursche T, Knoch KP, Schroeder M, Hoflack B, Solimena M.
Molecular & Cellular Proteomics 2008 Oct; 8(3):393.
Application：WB, Rat, INS-1 cells.
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ETD-Based Proteomic Profiling Improves Arginine Methylation Identification and Reveals Novel PRMT5 Substrates.
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