PPM1F 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: PPM1F transfected lysate ( 49.8 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
PPM1F transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PPM1F full-length
Host
Human
Theoretical MW (kDa)
50.05
Interspecies Antigen Sequence
Mouse (79); Rat (79)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PPM1F antibody (H00009647-B01) by Western Blots.
Western Blot
Lane 1: PPM1F transfected lysate ( 49.8 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
PPM1F transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PPM1F
Entrez GeneID
9647GeneBank Accession#
NM_014634Protein Accession#
NP_055449Gene Name
PPM1F
Gene Alias
CaMKPase, FEM-2, KIAA0015, POPX2, hFEM-2
Gene Description
protein phosphatase 1F (PP2C domain containing)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq
Other Designations
Ca(2+)/calmodulin-dependent protein kinase phosphatase|CaM-kinase phosphatase|PP2C phosphatase|partner of PIX 2|protein phosphatase 1F
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Interactome
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Disease
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