This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity:
Human
Quality Control Testing:
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of UBE2L6 transfected lysate using rabbit polyclonal anti-UBE2L6 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse polyclonal anti-UBE2L6.
Supplied Product:
Antibody pair set content: 1. Antibody pair for IP: rabbit polyclonal anti-UBE2L6 (300 ul) 2. Antibody pair for WB: mouse polyclonal anti-UBE2L6 (50 ul)
Storage Instruction:
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is highly similar in primary structure to the enzyme encoded by UBE2L3 gene. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations:
retinoic acid induced gene B protein,ubiquitin carrier protein,ubiquitin-protein ligase