RNH1 monoclonal antibody (M07), clone 3F5
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More Files
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Western Blot (Tissue lysate)
RNH1 monoclonal antibody (M07), clone 3F5. Western Blot analysis of RNH1 expression in human colon.
Western Blot (Cell lysate)
RNH1 monoclonal antibody (M07), clone 3F5. Western Blot analysis of RNH1 expression in K-562.
Western Blot (Transfected lysate)
Western Blot analysis of RNH1 expression in transfected 293T cell line by RNH1 monoclonal antibody (M07), clone 3F5.
Lane 1: RNH1 transfected lysate(50 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RNH1 is 10 ng/ml as a capture antibody.
Western Blot detection against Immunogen (76.45 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant RNH1.
Immunogen
RNH1 (AAH11500, 1 a.a. ~ 461 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSLDIQSLDIQCEELSDARWAELLPLLQQCQVVRLDDCGLTEARCKDISSALRVNPALAELNLRSNELGDVGVHCVLQGLQTPSCKIQKLSLQNCCLTGAGCGVLSSTLRTLPTLQELHLSDNLLGDAGLQLLCEGLLDPQCRLEKLQLEYCSLSAASCEPLASVLRAKPDFKELTVSNNDINEAGVRVLCQGLKDSPCQLEALKLESCGVTSDNCRDLCGIVASKASLRELALGSNKLGDVGMAELCPGLLHPSSRLRTLWIWECGITAKGCGDLCRVLRAKESLKELSLAGNELGDEGARLLCETLLEPGCQLESLWVKSCSFTAACCSHFSSVLAQNRFLLELQISNNRLEDAGVRELCQGLGQPGSVLRVLWLADCDVSDSSCSSLAATLLANHSLRELDLSNNCLGDAGILQLVESVRQPGCLLEQLVLYDIYWSEEMEDRLQALEKDKPSLRVIS
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (74); Rat (75)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (76.45 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
RNH1 monoclonal antibody (M07), clone 3F5. Western Blot analysis of RNH1 expression in human colon.Western Blot (Cell lysate)
RNH1 monoclonal antibody (M07), clone 3F5. Western Blot analysis of RNH1 expression in K-562.Western Blot (Transfected lysate)
Western Blot analysis of RNH1 expression in transfected 293T cell line by RNH1 monoclonal antibody (M07), clone 3F5.
Lane 1: RNH1 transfected lysate(50 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RNH1 is 10 ng/ml as a capture antibody.ELISA
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Gene Info — RNH1
Entrez GeneID
6050GeneBank Accession#
BC011500Protein Accession#
AAH11500Gene Name
RNH1
Gene Alias
MGC18200, MGC4569, MGC54054, RAI, RNH
Gene Description
ribonuclease/angiogenin inhibitor 1
Omim ID
173320Gene Ontology
HyperlinkOther Designations
OTTHUMP00000147628|Placental ribonuclease inhibitor|ribonuclease/angiogenin inhibitor
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Interactome
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Disease
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Publication Reference
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The ribonuclease/angiogenin inhibitor is also present in mitochondria and nuclei.
Furia A, Moscato M, Cali G, Pizzo E, Confalone E, Amoroso MR, Esposito F, Nitsch L, D'Alessio G.
FEBS Letters 2011 Feb; 585(4):613.
Application：IF, Human, HeLa cells.
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The ribonuclease/angiogenin inhibitor is also present in mitochondria and nuclei.
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