PPP3CA monoclonal antibody (M03), clone 2G8
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant PPP3CA.
Immunogen
PPP3CA (NP_000935, 1 a.a. ~ 84 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAP
Host
Mouse
Reactivity
Human, Rat
Interspecies Antigen Sequence
Mouse (100); Rat (100)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (34.98 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
PPP3CA monoclonal antibody (M03), clone 2G8. Western Blot analysis of PPP3CA expression in rat brain.Western Blot (Transfected lysate)
Western Blot analysis of PPP3CA expression in transfected 293T cell line by PPP3CA monoclonal antibody (M03), clone 2G8.
Lane 1: PPP3CA transfected lysate (Predicted MW: 57.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PPP3CA on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 ug/ml]ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PPP3CA on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PPP3CA
Entrez GeneID
5530GeneBank Accession#
NM_000944Protein Accession#
NP_000935Gene Name
PPP3CA
Gene Alias
CALN, CALNA, CALNA1, CCN1, CNA1, PPP2B
Gene Description
protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
Omim ID
114105Gene Ontology
HyperlinkGene Summary
catalytic subunit
Other Designations
calcineurin A alpha|protein phosphatase 3, catalytic subunit, alpha isoform|serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
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Interactomes
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Pathways
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Diseases
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Publication Reference
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No impact of protein phosphatases on connexin 43 phosphorylation in ischemic preconditioning.
Totzeck A, Boengler K, van de Sand A, Konietzka I, Gres P, Garcia-Dorado D, Heusch G, Schulz R.
American Journal of Physiology. Heart and Circulatory Physiology 2008 Oct; 295(5):H2106.
Application:WB-Ti, Pig, Pig left ventricle, cerebrum.
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No impact of protein phosphatases on connexin 43 phosphorylation in ischemic preconditioning.
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