SERPINE1 monoclonal antibody (M01), clone 3F2
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant SERPINE1.
Immunogen
SERPINE1 (NP_000593, 86 a.a. ~ 185 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAVDQLTR
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of SERPINE1 expression in transfected 293T cell line by SERPINE1 monoclonal antibody (M01), clone 3F2.
Lane 1: SERPINE1 transfected lysate(45.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of SERPINE1 transfected lysate using anti-SERPINE1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SERPINE1 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SERPINE1 is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — SERPINE1
Entrez GeneID
5054GeneBank Accession#
NM_000602Protein Accession#
NP_000593Gene Name
SERPINE1
Gene Alias
PAI, PAI-1, PAI1, PLANH1
Gene Description
serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Omim ID
173360Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
plasminogen activator inhibitor, type I|plasminogen activator inhibitor-1|serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
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Interactomes
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Pathways
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Diseases
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Publication Reference
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PAI-1 is a potential transcriptional silencer that supports bladder cancer cell activity.
Hideki Furuya, Yuka Sasaki, Runpu Chen, Rafael Peres, Kanani Hokutan, Kaoru Murakami, Nari Kim, Owen T M Chan, Ian Pagano, Lars Dyrskjøt, Jørgen B Jensen, Per-Uno Malmstrom, Ulrika Segersten, Yijun Sun, Abolfazl Arab, Hani Goodarzi, Steve Goodison, Charles J Rosser.
Scientific Reports 2022 Jul; 12(1):12186.
Application:ChIP-Seq, Human, RT-112, UM-UC-3 cells.
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Extracellular Matrix Remodeling by Bone Marrow Fibroblast-like Cells Correlates with Disease Progression in Multiple Myeloma.
Slany A, Haudek-Prinz V, Meshcheryakova A, Bileck A, Lamm W, Zielinski C, Gerner C, Drach J.
Journal of Proteome Research 2014 Feb; 13(2):844.
Application:WB-Ce, Human, Bone marrow fibroblast-like cells.
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PAI-1 is a potential transcriptional silencer that supports bladder cancer cell activity.
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