SMAD4 monoclonal antibody (M02), clone 3E2
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More Files
- More Functions
Western Blot (Cell lysate)
SMAD4 monoclonal antibody (M02), clone 3E2. Western Blot analysis of SMAD4 expression in K-562 ( Cat # L009V1 ).
Western Blot (Cell lysate)
SMAD4 monoclonal antibody (M02), clone 3E2. Western Blot analysis of SMAD4 expression in IMR-32 ( Cat # L008V1 ).
Western Blot (Cell lysate)
SMAD4 monoclonal antibody (M02), clone 3E2 Western Blot analysis of SMAD4 expression in Hela S3 NE ( Cat # L013V3 ).
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SMAD4 is approximately 3ng/ml as a capture antibody.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between CDKN1A and SMAD4. HeLa cells were stained with anti-CDKN1A rabbit purified polyclonal 1:1200 and anti-SMAD4 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunofluorescence
Immunofluorescence of monoclonal antibody to SMAD4 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (86.46 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant SMAD4.
Immunogen
SMAD4 (AAH02379, 1 a.a. ~ 552 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTSGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSSMMVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTANFPNIPVASTSQPASILGGSHSEGLLQIASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPHPGHYWPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (98); Rat (99)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (86.46 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
SMAD4 monoclonal antibody (M02), clone 3E2. Western Blot analysis of SMAD4 expression in K-562 ( Cat # L009V1 ).Western Blot (Cell lysate)
SMAD4 monoclonal antibody (M02), clone 3E2. Western Blot analysis of SMAD4 expression in IMR-32 ( Cat # L008V1 ).Western Blot (Cell lysate)
SMAD4 monoclonal antibody (M02), clone 3E2 Western Blot analysis of SMAD4 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SMAD4 is approximately 3ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between CDKN1A and SMAD4. HeLa cells were stained with anti-CDKN1A rabbit purified polyclonal 1:1200 and anti-SMAD4 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to SMAD4 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — SMAD4
Entrez GeneID
4089GeneBank Accession#
BC002379Protein Accession#
AAH02379Gene Name
SMAD4
Gene Alias
DPC4, JIP, MADH4
Gene Description
SMAD family member 4
Gene Ontology
HyperlinkGene Summary
mothers against decapentaplegic homolog 4|OTTHUMP00000163548|SMAD
Other Designations
MAD, mothers against decapentaplegic homolog 4|OTTHUMP00000163548|SMAD, mothers against DPP homolog 4|deleted in pancreatic carcinoma locus 4|mothers against decapentaplegic homolog 4|mothers against decapentaplegic, Drosophila, homolog of, 4
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Interactome
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Pathway
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Disease
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