Transient overexpression cell lysate was tested with Anti-HMGB2 antibody (H00003148-D01P) by Western Blots. SDS-PAGE Gel HMGB2 transfected lysate. Western Blot Lane 1: HMGB2 transfected lysate ( 24.00 KDa) Lane 2: Non-transfected lysate.
Storage Buffer:
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. [provided by RefSeq
Other Designations:
high-mobility group (nonhistone chromosomal) protein 2