GAPDH monoclonal antibody (M01A), clone 3C2
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More Files
- More Functions
Western Blot (Cell lysate)
GAPDH monoclonal antibody (M01A), clone 3C2 Western Blot analysis of GAPDH expression in A-431 ( Cat # L015V1 ).
Western Blot (Cell lysate)
GAPDH monoclonal antibody (M01A), clone 3C2. Western Blot analysis of GAPDH expression in HeLa ( Cat # L013V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of GAPDH expression in transfected 293T cell line by GAPDH monoclonal antibody (M01A), clone 3C2.
Lane 1: GAPDH transfected lysate(36.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (37.84 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant GAPDH.
Immunogen
GAPDH (NP_002037, 226 a.a. ~ 335 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Host
Mouse
Reactivity
Human, Mouse, Rat
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In ascites fluid
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
GAPDH monoclonal antibody (M01A), clone 3C2 Western Blot analysis of GAPDH expression in A-431 ( Cat # L015V1 ).Western Blot (Cell lysate)
GAPDH monoclonal antibody (M01A), clone 3C2. Western Blot analysis of GAPDH expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of GAPDH expression in transfected 293T cell line by GAPDH monoclonal antibody (M01A), clone 3C2.
Lane 1: GAPDH transfected lysate(36.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
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Gene Info — GAPDH
Entrez GeneID
2597GeneBank Accession#
NM_002046Protein Accession#
NP_002037Gene Name
GAPDH
Gene Alias
G3PD, GAPD, MGC88685
Gene Description
glyceraldehyde-3-phosphate dehydrogenase
Omim ID
138400Gene Ontology
HyperlinkGene Summary
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq
Other Designations
OTTHUMP00000174431|OTTHUMP00000174432|aging-associated gene 9 protein|glyceraldehyde 3-phosphate dehydrogenase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
- Metabolic pathways
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Disease
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Publication Reference
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Filamin A is reduced and contributes to the CASR sensitivity in human parathyroid tumors.
Mingione A, Verdelli C, Ferrero S, Vaira V, Guarnieri V, Scillitani A, Vicentini L, Balza G, Beretta E, Terranegra A, Vezzoli G, Soldati L, Corbetta S.
Journal of Molecular Endocrinology 2016 Nov; 58(2):91.
Application：WB-Tr, Human, HEK 293 cells .
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Filamin A is reduced and contributes to the CASR sensitivity in human parathyroid tumors.
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