FHL1 monoclonal antibody (M01), clone 2A9
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More Files
- More Functions
Western Blot (Cell lysate)
FHL1 monoclonal antibody (M01), clone 2A9. Western Blot analysis of FHL1 expression in NIH/3T3(Cat # L018V1 ).
Western Blot (Cell lysate)
FHL1 monoclonal antibody (M01), clone 2A9. Western Blot analysis of FHL1 expression in Raw 264.7.
Western Blot (Cell lysate)
FHL1 monoclonal antibody (M01), clone 2A9. Western Blot analysis of FHL1 expression in PC-12(Cat # L012V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of FHL1 expression in transfected 293T cell line by FHL1 monoclonal antibody (M01), clone 2A9.
Lane 1: FHL1 transfected lysate(31.9 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged FHL1 is approximately 0.3ng/ml as a capture antibody.
Western Blot detection against Immunogen (36.52 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant FHL1.
Immunogen
FHL1 (NP_001440, 23 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
DGHHCCLKCFDKFCANTCVECRKPIGADSKEVHYKNRFWHDTCFRCAKCLHPLANETFVAKDNKILCNKCTTREDSPKCKGCFKAIVAGDQNVEYKGT
Host
Mouse
Reactivity
Human, Mouse, Rat
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.52 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
FHL1 monoclonal antibody (M01), clone 2A9. Western Blot analysis of FHL1 expression in NIH/3T3(Cat # L018V1 ).Western Blot (Cell lysate)
FHL1 monoclonal antibody (M01), clone 2A9. Western Blot analysis of FHL1 expression in Raw 264.7.Western Blot (Cell lysate)
FHL1 monoclonal antibody (M01), clone 2A9. Western Blot analysis of FHL1 expression in PC-12(Cat # L012V1 ).Western Blot (Transfected lysate)
Western Blot analysis of FHL1 expression in transfected 293T cell line by FHL1 monoclonal antibody (M01), clone 2A9.
Lane 1: FHL1 transfected lysate(31.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged FHL1 is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — FHL1
Entrez GeneID
2273GeneBank Accession#
NM_001449Protein Accession#
NP_001440Gene Name
FHL1
Gene Alias
FHL1B, FLH1A, KYO-T, MGC111107, SLIM1, XMPMA, bA535K18.1
Gene Description
four and a half LIM domains 1
Omim ID
300163Gene Ontology
HyperlinkGene Summary
LIM proteins, named for 'LIN11, ISL1, and MEC3,' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM
Other Designations
Four-and-a-half LIM domains 1|OTTHUMP00000024097|bA535K18.1 (LIM protein SLIMMER)
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Interactome
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Publication Reference
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Carbonic Anhydrase III and Four-and-a-Half LIM Protein 1 are preferentially oxidized with muscle unloading.
Chen CN, Ferrington DA, Thompson LV.
Journal of Applied Physiology 2008 Aug; 105(5):1554.
Application：WB-Ti, Rat, Rat muscle tissue.
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Carbonic Anhydrase III and Four-and-a-Half LIM Protein 1 are preferentially oxidized with muscle unloading.
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