CTH monoclonal antibody (M03), clone S51
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More Files
- More Functions
Western Blot (Tissue lysate)
CTH monoclonal antibody (M03), clone S51. Western Blot analysis of CTH expression in mouse kidney.
Western Blot (Cell lysate)
CTH monoclonal antibody (M03), clone S51 Western Blot analysis of CTH expression in K-562 ( Cat # L009V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of CTH expression in transfected 293T cell line by CTH monoclonal antibody (M03), clone S51.
Lane 1: CTH transfected lysate(45 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CTH on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CTH is approximately 1ng/ml as a capture antibody.
Western Blot detection against Immunogen (70.07 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant CTH.
Immunogen
CTH (AAH15807, 1 a.a. ~ 405 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MQEKDASSQGFLPHFQHFATQAIHVGQDPEQWTSRAVVPPISLSTTFKQGAPGQHSGFEYSRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTITHLLKAGDQIICMDDVYGGTNRYFRQVASEFGLKISFVDCSKIKLLEAAITPETKLVWIETPTNPTQKVIDIEGCAHIVHKHGDIILVVDNTFMSPYFQRPLALGADISMYSATKYMNGRSDVVMGLVSVNCESLHNRLRFLQNSLGAVPSPIDCYLCNRGLKTLHVRMEKHFKNGMAVAQFLESNPWVEKVIYPGLPSHPQHELVKRQCTGCTGMVTFYIKGTLQHAEIFLKNLKLFTLAESLGGFESLAELPAIMTHASVLKNDRDVLGISDTLIRLSVGLEDEEDLLEDLDQALKAAHPPSGSHS
Host
Mouse
Reactivity
Human, Mouse
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (70.07 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
CTH monoclonal antibody (M03), clone S51. Western Blot analysis of CTH expression in mouse kidney.Western Blot (Cell lysate)
CTH monoclonal antibody (M03), clone S51 Western Blot analysis of CTH expression in K-562 ( Cat # L009V1 ).Western Blot (Transfected lysate)
Western Blot analysis of CTH expression in transfected 293T cell line by CTH monoclonal antibody (M03), clone S51.
Lane 1: CTH transfected lysate(45 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CTH on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CTH is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — CTH
Entrez GeneID
1491GeneBank Accession#
BC015807Protein Accession#
AAH15807Gene Name
CTH
Gene Alias
MGC9471
Gene Description
cystathionase (cystathionine gamma-lyase)
Gene Ontology
HyperlinkGene Summary
This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000010945|OTTHUMP00000010946|cystathionase|cysteine desulfhydrase|gamma-cystathionase|homoserine deaminase|homoserine dehydratase
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Interactome
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Pathway
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Disease
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Publication Reference
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Dysregulation of cystathionine γ-lyase promotes prostate cancer progression and metastasis.
Wang YH, Huang JT, Chen WL, Wang RH, Kao MC, Pan YR, Chan SH, Tsai KW, Kung HJ, Lin KT, Wang LH.
EMBO reports 2019 Oct; 20(10):e45986.
Application：IHC-P, WB-Ce, WB-Tr, Human, PC3, Bone metastasis-derived cells, Prostate tumor.
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Cystathionine-γ-lyase expression is associated with mitochondrial respiration during sepsis-induced acute kidney injury in swine with atherosclerosis.
Merz T, Wepler M, Nußbaum B, Vogt J, Calzia E, Wang R, Szabo C, Radermacher P, McCook O.
Intensive Care Medicine Experimental 2018 Oct; 6(1):43.
Application：IHC-P, Pig, Pig kidney.
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Relationship between selenoprotein P and selenocysteine lyase: Insights into selenium metabolism.
Seale LA, Ha HY, Hashimoto AC, Berry M.
Free Radical Biology & Medicine 2018 Mar; [Epub].
Application：WB, Human, Mouse, HepG2, Hepa1–6, Ht22 cells, brain, lungs, liver, kidneys, testes.
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The viability of primary hepatocytes is maintained under a low cysteine-glutathione redox state with a marked elevation in ophthalmic acid production.
Lee J, Sil Kang E, Kobayashi S, Homma T, Sato H, Geuk Seo H, Fujii J.
Experimental Cell Research 2017 Dec; 361(1):178.
Application：WB-Ce, Mouse, Mouse primary hepatocytes.
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Increased ophthalmic acid production is supported by amino acid catabolism under fasting conditions in mice.
Kobayashi S, Lee J, Takao T, Fujii J.
Biochemical and Biophysical Research Communications 2017 Jul; 491(3):649.
Application：WB-Ti, Mouse, Mouse liver.
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Ascorbic acid prevents acetaminophen-induced hepatotoxicity in mice by ameliorating glutathione recovery and autophagy.
Kurahashi T, Lee J, Nabeshima A, Homma T, Kang ES, Saito Y, Yamada S, Nakayama T, Yamada KI, Miyata S, Fujii J.
Archives of Biochemistry and Biophysics 2016 Aug; 604:36.
Application：WB-Ti, Mouse, Liver.
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Impacts of CD44 knockdown in cancer cells on tumor and host metabolic systems revealed by quantitative imaging mass spectrometry.
Ohmura M, Hishiki T, Yamamoto T, Nakanishi T, Kubo A, Tsuchihashi K, Tamada M, Toue S, Kabe Y, Saya H, Suematsu M.
Nitric Oxide : Biology and Chemistry 2015 Apr; 46:102.
Application：WB, Human, HCT116 cells.
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cGMP-Dependent Protein Kinase Contributes to Hydrogen Sulfide-Stimulated Vasorelaxation.
Bucci M, Papapetropoulos A, Vellecco V, Zhou Z, Zaid A, Giannogonas P, Cantalupo A, Dhayade S, Karalis KP, Wang R, Feil R, Cirino G.
PLoS One 2012 Dec; 7(12):e53319.
Application：WB-Ti, Mouse, Mouse aortas.
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Hydrogen sulfide impairs glucose utilization and increases gluconeogenesis in hepatocytes.
Zhang L, Yang G, Untereiner A, Ju Y, Wu L, Wang R.
Endocrinology 2012 Nov; 154(1):114.
Application：WB-Ce, Human, HepG2.
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An investigation of the mechanisms of hydrogen sulfide-induced vasorelaxation in rat middle cerebral arteries.
Streeter E, Hart J, Badoer E.
Naunyn-Schmiedeberg's Archives of Pharmacology 2012 Oct; 385(10):991.
Application：IF, IHC, Rat, Rat middle cerebral arteries.
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Characterization of Hydrogen Sulfide and Its Synthases, Cystathionine β-Synthase and Cystathionine γ-Lyase, in Human Prostatic Tissue and Cells.
Guo H, Gai JW, Wang Y, Jin HF, Du JB, Jin J.
Urology 2012 Feb; 79(2):483.
Application：IHC-Fr, WB-Ce, Human, Human prostate, LNCaP, PC-3, DU145, RWPE-2 cells.
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Glucocorticoids suppress cystathionine gamma-lyase expression and H(2)S production in lipopolysaccharide-treated macrophages.
Zhu XY, Liu SJ, Liu YJ, Wang S, Ni X.
Cellular and Molecular Life Sciences 2010 Apr; 67(7):1119.
Application：WB-Ce, WB-Tr, WB-Ti, Mouse, Macrophages, Brain, RAW 264.7 cells.
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H2S contributes to the hepatic arterial buffer response and mediates vasorelaxation of the hepatic artery via activation of KATP channels.
Siebert N, Cantre D, Eipel C, Vollmar B.
American Journal of Physiology. Gastrointestinal and Liver Physiology 2008 Oct; 295(6):G1266.
Application：IHC, WB-Ti, Rat, Rat liver and vascular .
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Dysregulation of cystathionine γ-lyase promotes prostate cancer progression and metastasis.
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