CDK5 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human CDK5 full-length ORF ( NP_004926.1, 1 a.a. - 292 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MQKYEKLEKIGEGTYGTVFKAKNRETHEIVALKRVRLDDDDEGVPSSALREICLLKELKHKNIVRLHDVLHSDKKLTLVFEFCDQDLKKYFDSCNGDLDPEIVKSFLFQLLKGLGFCHSRNVLHRDLKPQNLLINRNGELKLADFGLARAFGIPVRCYSAEVVTLWYRPPDVLFGAKLYSTSIDMWSAGCIFAELANAGRPLFPGNDVDDQLKRIFRLLGTPTEEQWPSMTKLPDYKPYPMYPATTSLVNVVPKLNATGRDLLQNLLKCNPVQRISAEEALQHPYFSDFCPP
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
57.75
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — CDK5
Entrez GeneID
1020GeneBank Accession#
NM_004935.1Protein Accession#
NP_004926.1Gene Name
CDK5
Gene Alias
PSSALRE
Gene Description
cyclin-dependent kinase 5
Omim ID
123831Gene Ontology
HyperlinkOther Designations
protein kinase CDK5 splicing
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Interactome
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Pathway
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Disease
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Publication Reference
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Cdk5-mediated phosphorylation of c-Myc on Ser-62 is essential in transcriptional activation of cyclin B1 by cyclin G1.
Seo HR, Kim J, Bae S, Soh JW, Lee YS.
The Journal of Biological Chemistry 2008 Apr; 283(23):15601.
Application:KA, WB, Human, NCI-H596 cells, Recombinant protein.
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Cdk5-mediated phosphorylation of c-Myc on Ser-62 is essential in transcriptional activation of cyclin B1 by cyclin G1.
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