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CCNE1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00000898-T02
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-CCNE1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 15.99
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CCNE1 antibody (H00000898-B03) by Western Blots.
    Western Blot
    QC Testing of H00000898-T02
    Lane 1: CCNE1 transfected lysate ( 45 KDa)
    Lane 2: Non-transfected lysate.
    SDS-PAGE Gel
    QC Testing of H00000898-T02
    CCNE1 transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 898
  • Protein Accession#:
  • -
  • Gene Name:
  • CCNE1
  • Gene Alias:
  • CCNE
  • Gene Description:
  • cyclin E1
  • Gene Summary:
  • The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq
  • Other Designations:
  • cyclin Es,cyclin Et
  • Interactome
  • Interactome
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