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ANK1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00000286-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-ANK1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 17.7
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ANK1 antibody (H00000286-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00000286-T01
    ANK1 transfected lysate.
    Western Blot
    QC Testing of H00000286-T01
    Lane 1: ANK1 transfected lysate ( 17.70 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 286
  • Gene Name:
  • ANK1
  • Gene Alias:
  • ANK,SPH1,SPH2
  • Gene Description:
  • ankyrin 1, erythrocytic
  • Gene Summary:
  • Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified. [provided by RefSeq
  • Other Designations:
  • ankyrin 1,ankyrin-1, erythrocytic,ankyrin-R
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