AGT monoclonal antibody (M01), clone 1A10
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More Files
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Western Blot (Cell lysate)
AGT monoclonal antibody (M01), clone 1A10 Western Blot analysis of AGT expression in HepG2 ( Cat # L019V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of AGT expression in transfected 293T cell line by AGT monoclonal antibody (M01), clone 1A10.
Lane 1: AGT transfected lysate(53 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged AGT is approximately 1ng/ml as a capture antibody.
Western Blot detection against Immunogen (37.73 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant AGT.
Immunogen
AGT (AAH11519, 376 a.a. ~ 485 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
TIHLTMPQLVLQGSYDLQDLLAQAELPAILHTELNLQKLSNDRIRVGEVLNSIFFELEADEREPTESTQQLNKPEVLEVTLNRPFLFAVYDQSATALHFLGRVANPLSTA
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Rat (62)
Isotype
IgG1 lambda
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
AGT monoclonal antibody (M01), clone 1A10 Western Blot analysis of AGT expression in HepG2 ( Cat # L019V1 ).Western Blot (Transfected lysate)
Western Blot analysis of AGT expression in transfected 293T cell line by AGT monoclonal antibody (M01), clone 1A10.
Lane 1: AGT transfected lysate(53 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged AGT is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — AGT
Entrez GeneID
183GeneBank Accession#
BC011519Protein Accession#
AAH11519Gene Name
AGT
Gene Alias
ANHU, FLJ92595, FLJ97926, SERPINA8
Gene Description
angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. [provided by RefSeq
Other Designations
OTTHUMP00000035878|alpha-1 antiproteinase, antitrypsin|angiotensin I|angiotensin II|angiotensinogen|pre-angiotensinogen|serine (or cysteine) proteinase inhibitor
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Interactome
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Pathway
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Disease
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Publication Reference
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A haplotype of human angiotensinogen gene containing -217A increases blood pressure in transgenic mice as compared to -217G.
Jain S, Vinukonda G, Fiering SN, Kumar A.
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology 2008 Oct; 295(6):R1849.
Application：WB, Mouse, Mouse plasma.
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HNF-1 alpha plays an important role in IL-6 induced expression of the Human Angiotensinogen Gene.
Jain S, Li Y, Patil S, Kumar A.
American Journal of Physiology. Cell Physiology 2007 May; 293(1):C401.
Application：WB, Human, Hep 3B2.1-7 (Hep 3B, Hep-3B, Hep3B).
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A haplotype of human angiotensinogen gene containing -217A increases blood pressure in transgenic mice as compared to -217G.
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