This DNA Ligation Kit enables ligation of cohesive end or blunt end DNA fragments within 5-30 minutes at 16 °C -25 °C. The DNA Ligation Kit is designed for fast and efficient ligation of both cohesive end and blunt ends. All necessary reagents required for ligation are provided. Simply ligation reaction can be started by adding 2x Ligation Buffer and Ligase Mixture to a mixture of vector and insert DNA solution. The ligation reaction mixture can be used directly for the transformation of chemical competent cells.
Quality Control Testing:
DNase: When 0.4 ug of lamda Hind III digest dephospholyrated by alkaline phosphatase was incubated with a mixture of 10 ul of the 2x Ligation Buffer and 1ul of the Ligase Mixture for 16 hours at 37°C, no degradation of the DNA fragment was observed on agarose gel electrophoresis. RNase: No RNase activity was detected by the use of RNaseAlert assay (Ambion).
Kit content 1.2x Ligation Buffer: Size 500 uL x 4 tubes 2. Ligase Mixture: Size 50 uL